Abstract
Proteomics is an indispensable tool for disease biomarker discovery. It is widely used for the analysis of biological fluids such as cerebrospinal fluid (CSF), blood, and saliva, which further aids in our understanding of disease incidence and progression. CSF is often the biospecimen of choice in case of intracranial tumors, as rapid changes in the tumor microenvironment can be easily assessed due to its close proximity to the brain. On the contrary studies comprising of serum or plasma samples do not truly reflect the underlying molecular alterations due to the presence of protective blood-brain barrier. We have described in here the detailed workflows for two advanced proteomics techniques, namely, 2D-DIGE (two-dimensional difference in-gel electrophoresis) and iTRAQ (isobaric tag for relative and absolute quantitation), for CSF analysis. Both of these techniques are very sensitive and widely used for quantitative proteomics analysis.
Aishwarya A. Rao and Kanika Mehta are the joint first authors.
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Acknowledgment
We would like to thank the High-Resolution Mass Spectrometry-Based Proteomics Research and Training Facility at IIT Bombay supported by the Department of Biotechnology (BT/PR13114/INF/22/206/2015) for processing the samples submitted for protein identification and quantification.
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Rao, A.A., Mehta, K., Gahoi, N., Srivastava, S. (2019). Application of 2D-DIGE and iTRAQ Workflows to Analyze CSF in Gliomas. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Cerebrospinal Fluid (CSF) Proteomics. Methods in Molecular Biology, vol 2044. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9706-0_6
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DOI: https://doi.org/10.1007/978-1-4939-9706-0_6
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