Abstract
Lipidomics aims at characterizing lipid profiles and their biological role with respect to protein expression involved in lipid metabolism. Specifically, cerebrospinal fluid (CSF) lipidomics is offering a new perspective in the search for surrogate biomarkers to facilitate early diagnosis of psychiatric and neurodegenerative diseases. In this chapter, we describe a nontargeted approach to profile lipid molecular species present in human CSF using ultrahigh-performance liquid chromatography–electrospray ionization–time-of-flight mass spectrometry (UPLC-ESI-ToF-MS). This workflow complements the toolbox useful for the exploration and monitoring neurodegenerative mechanisms associated with a dysregulation in lipid metabolism.
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Acknowledgments
The Proteomics Unit of Navarrabiomed is a member of Proteored, PRB3-ISCIII, and is supported by grant PT17/0019, of the PE I + D + i 2013–2016, funded by ISCIII and ERDF. Part of the work described here was funded by grants from the Spanish Ministry of Economy and Competitiveness (MINECO) (Ref. SAF2014-59340-R), Department of Economic Development from Government of Navarra (Ref. PC023-PC024, PC025, PC081-82 and PI059) and Obra Social la Caixa to E.S. The Clinical Neuroproteomics Laboratory of Navarrabiomed participates in the HUPO Brain Proteome Project and is lined up with the Spanish Initiative on the Human Proteome Project (SpHPP).
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Millán, L., Fernández-Irigoyen, J., Santamaría, E., Mayo, R. (2019). Mass Spectrometry Applied to Human Cerebrospinal Fluid Lipidome. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Cerebrospinal Fluid (CSF) Proteomics. Methods in Molecular Biology, vol 2044. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9706-0_23
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DOI: https://doi.org/10.1007/978-1-4939-9706-0_23
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