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Monitoring the Cerebrospinal Fluid Cytokine Profile Using Membrane-Based Antibody Arrays

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Cerebrospinal Fluid (CSF) Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2044))

Abstract

The brain is the most complex organ of the human body, and the study of the different diseases and injuries that affect it is far behind the ones that affect other organs. Some of these pathologies such as neurodegenerative diseases, physical injuries, and cancer present an important alteration in its inflammatory component, which affects their outcome in a positive or negative way. For this reason, it is important to characterize the joint expression of the cytokines and growth factors (GF) that are part of this inflammatory component. The cerebrospinal fluid (CSF) is in direct contact with the brain and spinal cord, being the best biofluid to study the cytokine and GF secretion patterns of these conditions. Currently, the proteomic workflows based on mass spectrometry (MS) are unable to easily detect these proteins in CSF. In this chapter, we describe a method based on cytokine membrane arrays to characterize, in a straightforward way, the secretion profile of different cytokines and GF at once in CSF.

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Acknowledgements

This work was funded by grants from the Spanish Ministry of Economy and Competitiveness (MINECO) (Ref. SAF2014-59340-R), Department of Economic Development from Government of Navarra (Ref. PC023-PC024, PC025, PC081-82 and PI059), and Obra Social la Caixa to ES. AGM was supported by PEJ-2014-A-61949 (MINECO) and by a predoctoral fellowship from the Public University of Navarra (UPNA). MLM is also supported by a predoctoral fellowship from UPNA. The Proteomics Unit of Navarrabiomed is a member of Proteored, PRB3-ISCIII, and is supported by grant PT17/0019, of the PE I+D+i 2013-2016, funded by ISCIII and ERDF. This project is part of the HUPO Brain Proteome Project and is lined up with the Spanish Initiative on the Human Proteome Project (SpHPP).

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Authors and Affiliations

  1. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Andrea González-Morales, Mercedes Lachén-Montes, Joaquín Fernández-Irigoyen & Enrique Santamaría

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  1. Andrea González-Morales
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  2. Mercedes Lachén-Montes
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  3. Joaquín Fernández-Irigoyen
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  4. Enrique Santamaría
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Correspondence to Enrique Santamaría .

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Editors and Affiliations

  1. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Enrique Santamaría

  2. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Joaquín Fernández-Irigoyen

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González-Morales, A., Lachén-Montes, M., Fernández-Irigoyen, J., Santamaría, E. (2019). Monitoring the Cerebrospinal Fluid Cytokine Profile Using Membrane-Based Antibody Arrays. In: Santamaría, E., Fernández-Irigoyen, J. (eds) Cerebrospinal Fluid (CSF) Proteomics. Methods in Molecular Biology, vol 2044. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9706-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9706-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9705-3

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