Abstract
Here we describe the use of a decellularized ECM produced in vitro by BALB/c 3T3 fibroblasts for the identification of ADAMTS substrates. Seeding of ADAMTS protease-producing HEK cells on top of the cell-free ECM followed by analysis of the conditioned medium by liquid chromatography tandem mass spectrometry (LC-MS/MS), allows for screening of ADAMTS substrates without prior purification of full-length protease.
Ruth Chiquet-Ehrismann was deceased at the time of publication.
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Acknowledgments
This work was supported by a grant from The Swiss National Science Foundation (SNF, Project funding in biology and medicine (division III)) grant no. 31003A_156740.
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Schnellmann, R., Chiquet-Ehrismann, R. (2020). Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_23
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DOI: https://doi.org/10.1007/978-1-4939-9698-8_23
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