Abstract
RNA in situ hybridization has an important place in matrix biology, as the only method that allows for in situ discrimination of precise spatial and temporal patterns of gene expression. Whereas immunohistochemistry shows where a matrix protein localizes, ISH identifies the cell of origin. Thus, these methods provide complementary information for insights on the life cycle of matrix molecules, including ADAMTS proteases. This protocol encompasses the staining of tissue sections to reveal expression of the gene of interest.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Lee D, Xiong S, Xiong WC (2013) General introduction to in situ hybridization protocol using nonradioactively labeled probes to detect mRNAs on tissue sections. Methods Mol Biol 1018:165–174
Chen CC, Wada K, Jarvis ED (2012) Radioactive in situ hybridization for detecting diverse gene expression patterns in tissue. J Vis Exp (62). https://doi.org/10.3791/3764.
Hurskainen TL, Apte SS (2010) In situ hybridization for metalloproteinases and their inhibitors. Methods Mol Biol 622:195–209
Wang F et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14(1):22–29
Mead TJ et al (2018) The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate, tendon-specific protective mechanism against heterotopic ossification. JCI Insight 3(7). https://doi.org/10.1172/jci.insight.92941
Cikach FS et al (2018) Massive aggrecan and versican accumulation in thoracic aortic aneurysm and dissection. JCI Insight 3(5). https://doi.org/10.1172/jci.insight.97167
Gueye NA et al (2017) Versican proteolysis by ADAMTS proteases and its influence on sex steroid receptor expression in uterine leiomyoma. J Clin Endocrinol Metab 102(5):1631–1641
Prins BP et al (2018) Exome-chip meta-analysis identifies novel loci associated with cardiac conduction, including ADAMTS6. Genome Biol 19(1):87
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Mead, T.J., Apte, S.S. (2020). Expression Analysis by RNAscope™ In Situ Hybridization. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9698-8_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9697-1
Online ISBN: 978-1-4939-9698-8
eBook Packages: Springer Protocols