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Stable Isotope-Resolved Metabolomics by NMR

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NMR-Based Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2037))

Abstract

Stable isotopes enable the tracing of atoms from precursors to products via metabolic transformations in situ and in crude extracts. NMR has some unique capabilities that are especially suited for metabolic analysis, including atom position-resolved isotope analysis and isotope editing. In this chapter we describe NMR-based analysis of positional isotopomer distribution using metabolic tracers.

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Abbreviations

DSS:

2,2-Dimethyl-2-silapentane-5-sulfonate

FID:

Free induction decay

rf:

Radiofrequency

RoI:

Region of interest

SIRM:

Stable isotope-resolved metabolomics

SNR:

Signal-to-noise ratio

TMS:

Tetramethylsilane

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Acknowledgments

This work was supported in part by NIH grants 1U24DK097215-01A1, CA163223-01A1, 5P20GM121327 (COBRE) the Carmen L. Buck endowment (to ANL), and the Edith D. Gardner Chair in Cancer Research (TWMF).

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Authors and Affiliations

  1. Center for Environmental and Systems Biochemistry, University of Kentucky, Lexington, KY, USA

    Penghui Lin, Andrew N. Lane & Teresa W.-M. Fan

  2. Department of Toxicology and Cancer Biology, University of Kentucky, Lexington, KY, USA

    Andrew N. Lane & Teresa W.-M. Fan

Authors
  1. Penghui Lin
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  2. Andrew N. Lane
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  3. Teresa W.-M. Fan
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Corresponding author

Correspondence to Andrew N. Lane .

Editor information

Editors and Affiliations

  1. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

    G. A. Nagana Gowda

  2. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Fred Hutch Cancer Research Center, Seattle, WA, USA

    Daniel Raftery

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Lin, P., Lane, A.N., Fan, T.WM. (2019). Stable Isotope-Resolved Metabolomics by NMR. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9690-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9689-6

  • Online ISBN: 978-1-4939-9690-2

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