Abstract
Laser capture microdissection has been around for almost a decade now. It has shown great promise for identification and determination of differences between activity and health of cells or even a single cell compared with its neighboring, local, or adjacent tissue inhabitants. Here we will provide a background to its use in neurological and cardiovascular fields and indicate a detailed methodological approach for successful RNA capture and analysis by one of the many current profiling systems available for pattern recognition.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Change history
24 December 2020
The original version of this chapter was revised. Dr. Anna Ludlaim has been included to the co-authors list.
References
Li Y, Zhong W, Jiang Z, Tang X (2019) New progress in the approaches for blood-brain barrier protection in acute ischemic stroke. Brain Res Bull 144:46–57. https://doi.org/10.1016/j.brainresbull.2018.11.006
Libby P (2007) Inflammatory mechanisms: the molecular basis of inflammation and disease. Nutr Rev 65(12 Pt 2):S140–S146
Ruan L, Wang B, ZhuGe Q, Jin K (2015) Coupling of neurogenesis and angiogenesis after ischemic stroke. Brain Res 1623:166–173. https://doi.org/10.1016/j.brainres.2015.02.042
Slevin M, Turu MM, Rovira N, Luque A, Baldellou M, Krupinski J, Badimon L (2010) Identification of a ‘snapshot’ of co-expressed angiogenic markers in laser-dissected vessels from unstable carotid plaques with targeted arrays. J Vasc Res 47(4):323–335. https://doi.org/10.1159/000265566
Moulton KS (2006) Angiogenesis in atherosclerosis: gathering evidence beyond speculation. Curr Opin Lipidol 17(5):548–555. https://doi.org/10.1097/01.mol.0000245261.71129.f0
Yin KJ, Hamblin M, Chen YE (2015) Angiogenesis-regulating microRNAs and ischemic stroke. Curr Vasc Pharmacol 13(3):352–365
Fend F, Raffeld M (2000) Laser capture microdissection in pathology. J Clin Pathol 53:666–672
Piao M, Tokunaga O (2006) Significant expression of endoglin (CD105), TGF beta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb 13:82–89
Norgen total RNA Purification Kit. https://norgenbiotek.com/product/total-rna-purification-plus-kit. Accessed 2 Dec 2019
Qubit RNA HS Assay Kit. https://www.thermofisher.com/order/catalog/product/Q32852. Accessed 2 Dec 2019
Agilent RNA 6000 Pico Kit. https://www.agilent.com/en/product/bioanalyzer-automated-electrophoresis/bioanalyzer-rna-kits-reagents/bioanalyzer-high-sensitivity-rna-analysis-228255#productdetails. Accessed 2 Dec 2019
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Slevin, M. et al. (2020). Laser-Capture Microdissection for Measurement of Angiogenesis After Stroke. In: Peplow, P.V., Martinez, B., Dambinova, S.A. (eds) Stroke Biomarkers. Neuromethods, vol 147. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9682-7_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9682-7_7
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9681-0
Online ISBN: 978-1-4939-9682-7
eBook Packages: Springer Protocols