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Measuring Protein Solubility

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Protein Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2039))

Abstract

Protein solubility determines the conditions under which the protein will remain in solution. As a result, it is an important quantity in applications that involve concentrated protein solutions. Here I describe the solubility measurement of the protein thaumatin in the presence of tartrate ions as a function of temperature. This method can be used to measure the solubility of other proteins.

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Acknowledgments

I gratefully acknowledge financial support from the National Science Foundation (DMR 0901260 and 1206416) and Yeshiva University.

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Authors and Affiliations

  1. Department of Physics, Yeshiva University, New York, NY, USA

    Neer Asherie

  2. Department of Biology, Yeshiva University, New York, NY, USA

    Neer Asherie

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  1. Neer Asherie
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Correspondence to Neer Asherie .

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Editors and Affiliations

  1. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland

    Jennifer J. McManus

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Asherie, N. (2019). Measuring Protein Solubility. In: McManus, J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9678-0_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9677-3

  • Online ISBN: 978-1-4939-9678-0

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