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Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis

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Book cover Imaging Gene Expression

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2038))

Abstract

One key step in the activation of inducible transcription factors is their homooligomerization, which can be measured in individual living cells by a fluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.

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Acknowledgments

We are grateful to Dr. Valeria Caiolfa and Dr. Moreno Zamai for their support on N&B analysis. Confocal imaging was carried out in ALEMBIC, an advanced microscopy laboratory established by IRCCS Ospedale San Raffaele and Università Vita-Salute San Raffaele. This work was supported by Fondazione Cariplo (E.C. and D.M.: 2014-1157).

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Authors and Affiliations

  1. Istituto Scientifico Ospedale San Raffaele, Centro di Imaging Sperimentale, Milan, Italy

    Eugenia Cammarota & Davide Mazza

  2. Fondazione CEN, European Center for Nanomedicine, Milan, Italy

    Eugenia Cammarota & Davide Mazza

Authors
  1. Eugenia Cammarota
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  2. Davide Mazza
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Corresponding author

Correspondence to Davide Mazza .

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Editors and Affiliations

  1. The Mina & Everard Goodman, Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan, Israel

    Yaron Shav-Tal

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Cammarota, E., Mazza, D. (2019). Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis. In: Shav-Tal, Y. (eds) Imaging Gene Expression. Methods in Molecular Biology, vol 2038. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9674-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9674-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9673-5

  • Online ISBN: 978-1-4939-9674-2

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