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Oligonucleotide-Based Therapies pp 173–186Cite as

Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2036))

Abstract

Oligonucleotides can be designed or evolved to bind to specific DNA, RNA, protein, or small molecule targets and thereby alter the biological function of the target. The therapeutic potential of oligonucleotides targeted to intracellular molecules will depend largely on their ability to be taken up by the cells of interest, as well as their subsequent subcellular distribution. Here we describe methods to characterize the extent and mechanism of cellular uptake of AS1411, an aptamer oligonucleotide that has progressed to human clinical trials and which is also widely used by researchers as a cancer-targeting ligand.

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Acknowledgments

The work described was funded by NIH grant R01CA122383 (Bates). The authors are named coinventors on issued patents or patent applications pertaining to AS1411

Author information

Authors and Affiliations

  1. Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Arizona College of Medicine, Tucson, AZ, USA

    Elsa M. Reyes-Reyes

  2. Department of Cellular and Molecular Medicine, University of Arizona Cancer Center, Tucson, AZ, USA

    Elsa M. Reyes-Reyes

  3. Department of Medicine, University of Louisville, Louisville, KY, USA

    Paula J. Bates

  4. Department of Biochemistry, University of Louisville, Louisville, KY, USA

    Paula J. Bates

  5. Experimental Therapeutics Program of the James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA

    Paula J. Bates

Authors
  1. Elsa M. Reyes-Reyes
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  2. Paula J. Bates
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Corresponding author

Correspondence to Paula J. Bates .

Editor information

Editors and Affiliations

  1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Olof Gissberg

  2. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Rula Zain

  3. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Karin E. Lundin

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Reyes-Reyes, E.M., Bates, P.J. (2019). Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer. In: Gissberg, O., Zain, R., Lundin, K. (eds) Oligonucleotide-Based Therapies. Methods in Molecular Biology, vol 2036. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9670-4_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9670-4_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9669-8

  • Online ISBN: 978-1-4939-9670-4

  • eBook Packages: Springer Protocols

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