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G-Quadruplex Nucleic Acids pp 87–103Cite as

Putting a New Spin of G-Quadruplex Structure and Binding by Analytical Ultracentrifugation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

Analytical ultracentrifugation is a powerful biophysical tool that provides information about G-quadruplex structure, stability, and binding reactivity. This chapter provides a simplified explanation of the method, along with examples of how it can be used to characterize G4 formation and to monitor small-molecule binding.

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Acknowledgments

This work was supported by NIH Grant GM077422 and by the James Graham Brown Foundation. We thank John Trent for his support and for helpful discussions.

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Authors and Affiliations

  1. James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA

    William L. Dean, Robert D. Gray, Lynn DeLeeuw, Robert C. Monsen & Jonathan B. Chaires

Authors
  1. William L. Dean
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  2. Robert D. Gray
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  3. Lynn DeLeeuw
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  4. Robert C. Monsen
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  5. Jonathan B. Chaires
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Corresponding author

Correspondence to Jonathan B. Chaires .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Dean, W.L., Gray, R.D., DeLeeuw, L., Monsen, R.C., Chaires, J.B. (2019). Putting a New Spin of G-Quadruplex Structure and Binding by Analytical Ultracentrifugation. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

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