Abstract
Protein–protein interactions and their dynamics define organelles’ structures and drive all aspects of cellular functions. In this chapter I describe a cross-linking mass spectrometry protocol that makes use of a newly developed MS-cleavable cross-linker to identify the protein pairs based on their proximity as defined by the length of the cross-linker. Unlike other approaches, such as immunoprecipitation-based interaction proteomics or yeast-two hybrid analysis, this protocol could characterize protein–protein interactions in a more physiological setting and allows the detection of native protein complexes contained in the mouse synaptosome.
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Liu, F. (2019). Analysis of Synaptic Protein–Protein Interaction by Cross-linking Mass Spectrometry. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 146. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9662-9_9
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DOI: https://doi.org/10.1007/978-1-4939-9662-9_9
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Publisher Name: Humana, New York, NY
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