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MALDI Imaging Mass Spectrometry: Neurochemical Imaging of Proteins and Peptides

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Book cover Neuroproteomics

Part of the book series: Neuromethods ((NM,volume 146))

Abstract

The central nervous system (CNS) constitutes the most intricate tissue in the human body. Neurological diseases, in particular, have a complex pathophysiology and are heterogeneous in their pathological and clinical presentation and therefore poorly understood on a molecular level. Increased insight in molecular CNS disease pathophysiology relates directly to the advancement of novel bioanalytical technologies that allow highly resolved, sensitive, specific, and comprehensive molecular analysis and molecular imaging in complex biological tissues, and in the CNS in particular. Imaging mass spectrometry (IMS) is an emerging technique for molecular imaging, characterized by its high molecular specificity and is therefore a powerful approach for investigating molecular localization patterns in CNS-derived tissue and cells. Over the last 20 years, IMS has been demonstrated to be a promising technology for chemical imaging in biochemical studies, but its application in clinical research is still in its infancy. The goal of this chapter is to provide the reader with a detailed step-by-step guide through the IMS workflow for the successful replication of published experimental data. Moreover, the aim is to give a concise overview of the major developments and applications of matrix-assisted laser desorption ionization (MALDI) based imaging mass spectrometry for neurochemical profiling with particular focus on protein and peptide imaging in neurodegenerative disease pathology.

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Acknowledgements

Funding was provided by The Swedish Research Council VR (no. 2014-6447 and no.2018-02181, J.H.; no. 2013-2546, H.Z.; no. 2017-00915, K.B.), Alzheimerfonden (J.H., K.B.), Hjärnfonden (K.B.), Åke Wiberg Stiftelse (J.H.), Ahlén Stiftelsen (J.H.), Stiftelsen Gamla Tjänarinnor (J.H., H.Z., K.B.), Torsten Söderberg Foundation (K.B.), the Knut and Alice Wallenberg Foundation (H.Z.), and the European Research Council (681712, H.Z.).

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Authors and Affiliations

  1. Department of Psychiatry and Neurochemistry, Mölndal Hospital, Sahlgrenska Academy at the University of Gothenburg, Mölndal, Sweden

    Jörg Hanrieder, Henrik Zetterberg & Kaj Blennow

  2. Department of Neurodegenerative Disease, Queen Square Institute of Neurology, University College London, London, UK

    Jörg Hanrieder & Henrik Zetterberg

  3. UK Dementia Research Institute at UCL, London, UK

    Henrik Zetterberg

  4. Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital Mölndal, Mölndal, Sweden

    Henrik Zetterberg & Kaj Blennow

Authors
  1. Jörg Hanrieder
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  2. Henrik Zetterberg
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  3. Kaj Blennow
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Correspondence to Jörg Hanrieder .

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Editors and Affiliations

  1. Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

    Ka Wan Li

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Hanrieder, J., Zetterberg, H., Blennow, K. (2019). MALDI Imaging Mass Spectrometry: Neurochemical Imaging of Proteins and Peptides. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 146. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9662-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9662-9_15

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  • Publisher Name: Humana, New York, NY

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