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2D-DIGE as a Tool in Neuroproteomics

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Neuroproteomics

Part of the book series: Neuromethods ((NM,volume 146))

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Abstract

Neuroproteomics encompasses the study of all protein-related dynamics of the nervous system, not only on a morphological level in development and disease but also functional aspects of the process of learning as well as changes in clinical conditions like depression and addiction. Detection of these changes in protein abundance under defined conditions is the field of differential proteome analysis, with two-dimensional difference gel electrophoresis (2D-DIGE) currently being the most comprehensive technique to quantify these changes while retaining information about isoforms and posttranslational modifications. This chapter focusses on technical aspects of 2D-DIGE as well as a brief overview of successful applications of this technique in neuroproteomics.

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Acknowledgments

The author wants to thank Maithili Shroff for proof-reading the manuscript.

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Authors and Affiliations

  1. Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, Sir James Black Centre, School of Life Sciences, University of Dundee, Dundee, UK

    Florian Weiland

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  1. Florian Weiland
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Correspondence to Florian Weiland .

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Editors and Affiliations

  1. Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

    Ka Wan Li

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Weiland, F. (2019). 2D-DIGE as a Tool in Neuroproteomics. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 146. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9662-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9662-9_13

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  • Publisher Name: Humana, New York, NY

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