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Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models

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Microglia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2034))

Abstract

During insults and disease blood-borne monocytes can invade brain and spinal cord, contributing to the neuroimmune response together with brain-resident microglia. The specific function of brain-infiltrating monocytes has been difficult to ascertain because of shared marker expression and morphology of these two immune cell types. Here we describe our method of repopulating the brain with circulating monocytes after microglia ablation to investigate the physiology of brain-invading monocytes, which engraft under these conditions.

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Acknowledgments

This work was supported in part by Emory University Pediatric Flow Cytometry Core, the Emory University Research Committee, the National Institutes of Health, the Office of the Director, and the National Institute of Neurological Disorders and Stroke Grant R01 NS097776.

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Authors and Affiliations

  1. Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA, USA

    Nicholas H. Varvel

  2. Third Rock Ventures, Boston, MA, USA

    Richard M. Ransohoff

  3. Department of Cell Biology, Harvard Medical School, Boston, MA, USA

    Richard M. Ransohoff

  4. German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany

    Jonas J. Neher

  5. Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany

    Jonas J. Neher

Authors
  1. Nicholas H. Varvel
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  2. Richard M. Ransohoff
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  3. Jonas J. Neher
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Corresponding author

Correspondence to Nicholas H. Varvel .

Editor information

Editors and Affiliations

  1. Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

    Olga Garaschuk

  2. Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

    Alexei Verkhratsky

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Varvel, N.H., Ransohoff, R.M., Neher, J.J. (2019). Functional Analysis of Brain-Engrafted Monocytes After Microglia Ablation in Mouse Models. In: Garaschuk, O., Verkhratsky, A. (eds) Microglia. Methods in Molecular Biology, vol 2034. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9658-2_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9658-2_22

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9657-5

  • Online ISBN: 978-1-4939-9658-2

  • eBook Packages: Springer Protocols

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