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Long-Term In Vivo Imaging of Individual Microglial Cells

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Microglia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2034))

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Abstract

Microglia are morphologically dynamic cells, neatly arranged in an interconnected three-dimensional lattice throughout the brain, constantly surveying the parenchyma, and swiftly responding to a variety of external stimuli. Capturing the dynamics of their morphology, reaction to trauma, pathogens, or endogenous stimuli, and studying changes in their network in their physiological environment requires the use of two-photon microscopy, as well as a precise repositioning strategy. Herein, we describe a robust repeatable localization method, coupled with optimized in vivo two-photon microscopy for long-term imaging of single microglia cells in the mouse brain.

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Acknowledgments

The authors would like to express their sincere thanks to the people who have contributed toward developing and optimizing the methods described herein; particularly David Milford, Daniel Eicke, and Christian Feldhaus for developing and testing the Head Fixation system. The support of Professor Mathias Jucker throughout the development of this work is also greatly appreciated.

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Authors and Affiliations

  1. German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany

    Angelos A. Skodras & Jonas J. Neher

  2. Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany

    Angelos A. Skodras & Jonas J. Neher

  3. Institute of Cell Biology and Neuroscience, University of Frankfurt, Frankfurt am Main, Germany

    Jasmin K. Hefendehl

  4. Buchmann Institute for Molecular Life Sciences, University of Frankfurt, Frankfurt am Main, Germany

    Jasmin K. Hefendehl

Authors
  1. Angelos A. Skodras
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  2. Jasmin K. Hefendehl
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  3. Jonas J. Neher
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Corresponding author

Correspondence to Angelos A. Skodras .

Editor information

Editors and Affiliations

  1. Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

    Olga Garaschuk

  2. Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

    Alexei Verkhratsky

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Skodras, A.A., Hefendehl, J.K., Neher, J.J. (2019). Long-Term In Vivo Imaging of Individual Microglial Cells. In: Garaschuk, O., Verkhratsky, A. (eds) Microglia. Methods in Molecular Biology, vol 2034. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9658-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9658-2_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9657-5

  • Online ISBN: 978-1-4939-9658-2

  • eBook Packages: Springer Protocols

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