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Immunophenotyping of Acute Myeloid Leukemia

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Immunophenotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2032))

Abstract

Immunophenotyping by multiparameter flow cytometry is a rapid and efficient technique to simultaneously assess and correlate multiple individual cell properties like size and internal complexity along with antigen expression in a population of cells. This method is utilized for rapid characterization of the blasts and classification of acute myeloid leukemia (AML), in both the peripheral blood (PB) and bone marrow (BM). This technique is not only useful in the initial diagnosis but also in monitoring and determining prognosis of the disease through minimal residual disease (MRD) testing. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes the principles of data analysis for AML classification and MRD testing.

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Acknowledgments

This research was supported by the Intramural Research Program of the National Institutes of Health.

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Authors and Affiliations

  1. Hematology Section, Department of Laboratory Medicine, Clinical Center, National Institutes of Health (NIH), Bethesda, MD, USA

    Pallavi Kanwar Galera, Chunjie Jiang & Raul Braylan

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  1. Pallavi Kanwar Galera
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  2. Chunjie Jiang
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  3. Raul Braylan
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Correspondence to Pallavi Kanwar Galera .

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Editors and Affiliations

  1. Frederick, MD, USA

    J. Philip McCoy, Jr

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Galera, P.K., Jiang, C., Braylan, R. (2019). Immunophenotyping of Acute Myeloid Leukemia. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9650-6_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9649-0

  • Online ISBN: 978-1-4939-9650-6

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