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Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles

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Immunophenotyping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2032))

Abstract

Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.

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Author information

Authors and Affiliations

  1. Department of Inflammation and Immunity, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA

    Nicholas Wanner & Kewal Asosingh

Authors
  1. Nicholas Wanner
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  2. Kewal Asosingh
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Corresponding author

Correspondence to Kewal Asosingh .

Editor information

Editors and Affiliations

  1. Frederick, MD, USA

    J. Philip McCoy, Jr

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Wanner, N., Asosingh, K. (2019). Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9650-6_12

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9649-0

  • Online ISBN: 978-1-4939-9650-6

  • eBook Packages: Springer Protocols

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