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The Comet Assay in Marine Animals

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Genotoxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2031))

Abstract

Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows for the detection of DNA single- and double-strand breaks, as well as the presence of alkali labile sites and cross-links. Here we describe protocols for the single-cell gel electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH = 12.1) and neutral (pH = 8) versions when applied in marine animals.

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Authors and Affiliations

  1. Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy

    Patrizia Guidi & Giada Frenzilli

  2. CEFAS Laboratory, Weymouth, Dorset, UK

    Brett P. Lyons

Authors
  1. Patrizia Guidi
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  2. Brett P. Lyons
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  3. Giada Frenzilli
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Corresponding author

Correspondence to Giada Frenzilli .

Editor information

Editors and Affiliations

  1. CSIR—Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India

    Alok Dhawan

  2. New Delhi, Delhi, India

    Mahima Bajpayee

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Guidi, P., Lyons, B.P., Frenzilli, G. (2019). The Comet Assay in Marine Animals. In: Dhawan, A., Bajpayee, M. (eds) Genotoxicity Assessment. Methods in Molecular Biology, vol 2031. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9646-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9646-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9645-2

  • Online ISBN: 978-1-4939-9646-9

  • eBook Packages: Springer Protocols

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