Abstract
UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
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Acknowledgments
W. v. H. acknowledges a NWO research grant (no. 2014-01- 124PRO).
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van Helmond, W., de Puit, M. (2019). Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS. In: Alterman, M. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 2030. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_33
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DOI: https://doi.org/10.1007/978-1-4939-9639-1_33
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9639-1
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