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High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization

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Amino Acid Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2030))

Abstract

d-Amino acids have recently attracted much attention in various research fields including medical, clinical, and food industry due to their important biological functions that differ from l-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 d-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan

    Yosuke Nakano, Moyu Taniguchi, Yutaka Umakoshi, Daisuke Watai & Eiichiro Fukusaki

Authors
  1. Yosuke Nakano
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  2. Moyu Taniguchi
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  3. Yutaka Umakoshi
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  4. Daisuke Watai
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  5. Eiichiro Fukusaki
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Corresponding author

Correspondence to Eiichiro Fukusaki .

Editor information

Editors and Affiliations

  1. Office of Policy for Pharmaceutical Quality Office of Pharmaceutical Quality, Center for Drug Evaluation and Research US FDA, Silver Spring, MD, USA

    Michail A. Alterman

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Nakano, Y., Taniguchi, M., Umakoshi, Y., Watai, D., Fukusaki, E. (2019). High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization. In: Alterman, M. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 2030. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9639-1_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9638-4

  • Online ISBN: 978-1-4939-9639-1

  • eBook Packages: Springer Protocols

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