Abstract
Recombinant proteins are essential components of therapeutic, biotechnological, food, and household products. In some cases, recombinant proteins must be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrometry (HRMS) analysis of the hydrolytic products. Absolute quantification is obtained by adding controlled amounts of labeled amino acids that serve as standards.
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Louwagie, M., Kieffer-Jaquinod, S., Brun, V. (2019). Ultrasensitive Quantification of Recombinant Proteins Using AAA-MS. In: Alterman, M. (eds) Amino Acid Analysis. Methods in Molecular Biology, vol 2030. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9639-1_1
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DOI: https://doi.org/10.1007/978-1-4939-9639-1_1
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Online ISBN: 978-1-4939-9639-1
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