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Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene

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Antiviral Resistance in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2028))

Abstract

NB-LRR class plant virus resistance gene is a one of the key players that shape the plant–virus interaction. Evolutionary arms race between plants and viruses often results in the breakdown of virus resistance in plants, which leads to a disastrous outcome in agricultural production. Although studies have analyzed the nature of plant virus resistance breakdown, it is still difficult to foresee the breakdown of a given virus resistance gene. In this chapter, we provide a protocol for evaluating the durability of plant virus resistance gene, which comprises the random mutagenesis of a virus gene, the introduction of the mutagenized gene into a virus context with highly efficient inoculation system, and the efficient screening of virus mutants that can overcome or escape a virus resistance.

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Acknowledgments

This work was supported in part by JSPS KAKENHI Grant Numbers 21380032, 24658044, 26292026, and 15K14664.

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Authors and Affiliations

  1. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan

    Kengo Idehara, Masamichi Nishiguchi & Kappei Kobayashi

  2. Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan

    Reiko Tomita & Ken-Taro Sekine

Authors
  1. Kengo Idehara
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  2. Reiko Tomita
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  3. Ken-Taro Sekine
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  4. Masamichi Nishiguchi
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  5. Kappei Kobayashi
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Corresponding author

Correspondence to Kappei Kobayashi .

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Editors and Affiliations

  1. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan

    Kappei Kobayashi

  2. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan

    Masamichi Nishiguchi

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Idehara, K., Tomita, R., Sekine, KT., Nishiguchi, M., Kobayashi, K. (2019). Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene. In: Kobayashi, K., Nishiguchi, M. (eds) Antiviral Resistance in Plants. Methods in Molecular Biology, vol 2028. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9635-3_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9635-3_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9634-6

  • Online ISBN: 978-1-4939-9635-3

  • eBook Packages: Springer Protocols

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