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Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana

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Book cover Antiviral Resistance in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2028))

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Abstract

Antiviral RNA silencing and the resistance gene-conferred defense response are major antiviral immune systems in plants. Several of the components involved have been genetically or biochemically identified in Arabidopsis thaliana. One powerful tool to dissect antiviral immune systems involves a reverse genetic approach that analyzes Arabidopsis mutant lines with impaired antiviral defense responses. In particular, to better understand the signaling networks involved in the resistance gene-conferred antiviral response in host plants, establishment of mutant lines carrying the homozygous mutant allele and antiviral resistance gene is required. The information on well-characterized defense-related signaling mutant alleles and the PCR-based genotyping method provided in this chapter allows the efficient selection of Arabidopsis mutant lines that can be used to study antiviral resistance signaling networks and resistance mechanisms.

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Acknowledgments

This work was supported by grants for JSPS Research Fellows (15J01964), for “Scientific Research on Innovative Areas” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (Grant numbers: 16H06429, 16K21723, and 16H06435), and by the Japan Society for the Promotion of Science (JSPS) through the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of International Agricultural Immunology Research-Core for Quantum Improvement in Food Safety.”

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  1. Graduate School of Agricultural Science, Tohoku University, Sendai, Japan

    Yukiyo Sato & Hideki Takahashi

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  1. Yukiyo Sato
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  2. Hideki Takahashi
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Correspondence to Hideki Takahashi .

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  1. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan

    Kappei Kobayashi

  2. Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan

    Masamichi Nishiguchi

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Sato, Y., Takahashi, H. (2019). Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana. In: Kobayashi, K., Nishiguchi, M. (eds) Antiviral Resistance in Plants. Methods in Molecular Biology, vol 2028. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9635-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9635-3_3

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