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Isolation and Characterization of Cardiac Progenitor Cells

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Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2029))

Abstract

Cardiac progenitor cells (CPCs) are gaining interest as a therapeutic option for the treatment of the heart. Due to the limited pool of CPCs residing in the heart, it is essential to isolate and expand the CPCs in vitro. Here we describe the protocol for isolation and culture of the heterogeneous population of CPCs from right atrial appendage and left ventricular tissue collected from patients undergoing on-pump coronary artery bypass graft surgery for the treatment of ischemic heart disease. Our protocol is developed to simultaneously isolate, culture, and characterize the CPCs from both atrial and ventricular tissues. We also describe the protocol for flow cytometry and immunohistochemical characterization of the isolated CPCs.

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Author information

Authors and Affiliations

  1. Department of Physiology-HeartOtago, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand

    Parul Dixit & Rajesh G. Katare

Authors
  1. Parul Dixit
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  2. Rajesh G. Katare
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Corresponding author

Correspondence to Rajesh G. Katare .

Editor information

Editors and Affiliations

  1. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Mugdha V. Joglekar

  2. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Anandwardhan A. Hardikar

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Dixit, P., Katare, R.G. (2019). Isolation and Characterization of Cardiac Progenitor Cells. In: Joglekar, M., Hardikar, A. (eds) Progenitor Cells. Methods in Molecular Biology, vol 2029. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9631-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9631-5_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9630-8

  • Online ISBN: 978-1-4939-9631-5

  • eBook Packages: Springer Protocols

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