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Rapid Detection of Hormonal Involvement in Light Responses

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Phytochromes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2026))

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Abstract

Many aspects of light-controlled metabolism and development of plants depend on hormonal pathways. Here, a method is described to identify such hormonal dependence in light-regulated processes. A number of compounds—hormones and chemicals which interfere with hormonal pathways—are listed because of their usefulness in pharmacological treatment experiments. As an example for practical use of such compounds, elongation growth is discussed. An experimental setup is described in which plants are grown so that their structures develop predominantly in a two-dimensional plane. Time-lapse imaging is used to follow the plants in time, and image analysis reveals changes in plant morphology.

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Author information

Authors and Affiliations

  1. Laboratory for Functional Plant Biology, Department of Biology, Ghent University, Ghent, Belgium

    Filip Vandenbussche

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  1. Filip Vandenbussche
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Correspondence to Filip Vandenbussche .

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Editors and Affiliations

  1. Faculty of Biology, Institute of Biology II and Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany

    Andreas Hiltbrunner

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Vandenbussche, F. (2019). Rapid Detection of Hormonal Involvement in Light Responses. In: Hiltbrunner, A. (eds) Phytochromes. Methods in Molecular Biology, vol 2026. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9612-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9612-4_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9611-7

  • Online ISBN: 978-1-4939-9612-4

  • eBook Packages: Springer Protocols

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