Abstract
Flagella are effective organelles of locomotion and one of several virulence factors in Proteus mirabilis. To study their properties and role in virulence, we describe a protocol to extract and purify the native flagellin of P. mirabilis. Purified flagellin can be visualized by SDS-PAGE or immunoblot and is suitable for downstream applications such as immunization.
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González, M.J., Iribarnegaray, V., Zunino, P., Scavone, P. (2019). Purification of Native Flagellin. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_5
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DOI: https://doi.org/10.1007/978-1-4939-9601-8_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9600-1
Online ISBN: 978-1-4939-9601-8
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