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Proteus mirabilis pp 129–137Cite as

Adherence of Proteus mirabilis to Uroepithelial Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2021))

Abstract

Bacterial adherence to eukaryotic cells is mediated by different adhesins that can act at different stages in bacteria–host interaction. Abundant evidence has suggested that adherence is critical for infection by bacterial pathogens. Proteus mirabilis is an opportunistic pathogen which frequently infects the human urinary tract, particularly in patients with indwelling urinary catheters. Sequencing of the genome of this pathogen has revealed the existence of a remarkable amount of complete fimbrial operons. In this chapter, we describe in vitro adherence assays of P. mirabilis to uroepithelial cells, which can provide relevant results to assess virulence of uropathogenic strains.

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References

  1. Rendón MA, Saldaña Z, Erdem AL, Monteiro-Neto V, Vázquez A, Kaper JB, Puente JL, Girón JA (2007) Commensal and pathogenic Escherichia coli use a common pilus adherence factor for epithelial cell colonization. Proc Natl Acad Sci U S A 104(25):10637–10642

    Article  Google Scholar 

  2. Brumell JH, Finlay BB (2000) Bacterial adherence, colonization, and invasion of mucosal surfaces. In: Brogden K, Roth J, Stanton T, Bolin C, Minion F, Wannemuehler M (eds) Virulence mechanisms of bacterial pathogens, 3rd edn. ASM Press, Washington, DC, pp 3–17

    Chapter  Google Scholar 

  3. Jonson AB, Normak S, Rhen M (2005) Fimbriae, pili, flagella and bacterial virulence. In: Russell W, Herwarld H (eds) Concepts in bacterial virulence, vol 12. Karger Publishers, Basel, pp 67–89

    Google Scholar 

  4. Klemm P, Schembri M (2004) Type 1 fimbriae, curli, and antigen 43: adhesion, colonization, and biofilm formation. EcoSal Plus 1(1). https://doi.org/10.1128/ecosalplus.8.3.2.6

  5. Scavone P, Iribarnegaray V, Caetano AL, Schlapp G, Härtel S, Zunino P (2016) Fimbriae have distinguishable roles in Proteus mirabilis biofilm formation. Pathog Dis 74(5):pii: ftw033. https://doi.org/10.1093/femspd/ftw033

    Article  CAS  Google Scholar 

  6. Scavone P, Villar S, Umpiérrez A, Zunino P (2015) Role of Proteus mirabilis MR/P fimbriae and flagella in adhesion, cytotoxicity and genotoxicity induction in T24 and Vero cells. Pathog Dis 73:pii: ftv017. https://doi.org/10.1093/femspd/ftv017

    Article  CAS  Google Scholar 

  7. Armbruster CE, Mobley HLT (2012) Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis. Nat Rev Microbiol 10(11):743–754

    Article  CAS  Google Scholar 

  8. Nielubowicz GR, Mobley HLT (2010) Host-pathogen interactions in urinary tract infection. Nat Rev Urol 7(8):430–441

    Article  CAS  Google Scholar 

  9. Alamuri P, Löwer M, Hiss JA, Himpsl SD, Schneider G, Mobley HLT (2010) Adhesion, invasion, and agglutination mediated by two trimeric autotransporters in the human uropathogen Proteus mirabilis. Infect Immun 78(11):4882–4894

    Article  CAS  Google Scholar 

  10. Pearson MM, Sebaihia M, Churcher C, Quail MA, Seshasayee AS, Luscombe NM, Abdellah Z, Arrosmith C, Atkin B, Chillingworth T, Hauser H, Jagels K, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Walker D, Whithead S, Thomson NR, Rather PN, Parkhill J, Mobley HL (2008) Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility. J Bacteriol 190(11):4027–4037

    Article  CAS  Google Scholar 

  11. Freshney RI (1987) Culture of animal cells: a manual of basic technique, 2nd edn. Alan R. Liss, New York

    Google Scholar 

  12. Bruce AW, Chan RC, Pinkerton D, Morales A, Chadwick P (1983) Adherence of gram-negative uropathogens to human uroepithelial cells. J Urol 130(2):293–298

    Article  CAS  Google Scholar 

  13. Shih C, Weinberg RA (1982) Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell 29(1):161–169

    Article  CAS  Google Scholar 

  14. Wray S, Hull S, Cook J, Barrish J, Hull R (1986) Identification and characterization of urinary cell adhesin from a uropathogenic isolate of Proteus mirabilis. Infect Immun 54(1):43–49

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Zunino P, Sosa V, Allen AG, Preston A, Schlapp G, Maskell DJ (2003) Proteus mirabilis fimbriae (PMF) are important for both bladder and kidney colonization in mice. Microbiology 149(11):3231–3237

    Article  CAS  Google Scholar 

  16. Latta RK, Schur MJ, Tolson DL, Altman E (1998) The effect of growth conditions on in vitro adherence, invasion, and NAF expression by Proteus mirabilis 7570. Can J Microbiol 44(9):896–904

    Article  CAS  Google Scholar 

  17. Bahrani FK, Mobley HLT (1994) Proteus mirabilis MR/P fimbrial operon: genetic organization, nucleotide sequence, and conditions for expression. J Bacteriol 176(11):3412–3419

    Article  CAS  Google Scholar 

  18. Szabo RJ, Shortliffe LM, Stamey TA (1987) Adherence of Escherichia coli and Proteus mirabilis to human transitional cells. J Urol 137(4):793–797

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Departamento de MicrobiologÚa, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay

    María José González, Victoria Iribarnegaray, Paola Scavone & Pablo Zunino

Authors
  1. María José González
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  2. Victoria Iribarnegaray
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  3. Paola Scavone
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  4. Pablo Zunino
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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González, M.J., Iribarnegaray, V., Scavone, P., Zunino, P. (2019). Adherence of Proteus mirabilis to Uroepithelial Cells. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9601-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9600-1

  • Online ISBN: 978-1-4939-9601-8

  • eBook Packages: Springer Protocols

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