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Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification

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Immunoproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2024))

Abstract

The study of the humoral immune response to infectious and chronic diseases is important for understanding the disease progression, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example.

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Acknowledgments

This work was supported by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Contract No. HHSN266200500041C, and in part by the National Research Council, Canada. The authors thank Dr Freyja Lynn, Vicki Pierson, Kristin Debord, and Patrick Sanz (National Institutes of Allergy and Infectious Diseases); Dr J. Wayne Conlan, Luc Tessier, and Marianne Savicky (National Research Council, Canada); and Gretchen Stup (DynPort Vaccine Company) for their contributions throughout this work.

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Authors and Affiliations

  1. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada

    Kelly M. Fulton, Anna Ananchenko & Susan M. Twine

  2. Dynport Vaccine Company LLC, Frederick, MD, USA

    Lawrence Wolfraim & Shannon Martin

Authors
  1. Kelly M. Fulton
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  2. Anna Ananchenko
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  3. Lawrence Wolfraim
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  4. Shannon Martin
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  5. Susan M. Twine
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Corresponding author

Correspondence to Kelly M. Fulton .

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Editors and Affiliations

  1. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada

    Kelly M. Fulton

  2. Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada

    Susan M. Twine

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Fulton, K.M., Ananchenko, A., Wolfraim, L., Martin, S., Twine, S.M. (2019). Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification. In: Fulton, K., Twine, S. (eds) Immunoproteomics. Methods in Molecular Biology, vol 2024. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9597-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9597-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9596-7

  • Online ISBN: 978-1-4939-9597-4

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