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Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins

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Vaccinia Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

Vaccinia virus recombinants that express fluorescent proteins have a variety of applications such as the identification of infected cells, efficient screening for genetically modified strains, and molecular characterization of virus replication and spread. The detection of fluorescent proteins and viral–fluorescent fusion proteins by fluorescence microscopy is noninvasive and can be used to describe protein localization in live cells and track the intracellular movement of virus particles. This chapter describes a number of approaches for the construction of plasmids and subsequent generation and isolation of fluorescent recombinant viruses.

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Author information

Authors and Affiliations

  1. The University of Sydney, School of Life and Environmental Sciences, Sydney, NSW, Australia

    N. Bishara Marzook & Timothy P. Newsome

Authors
  1. N. Bishara Marzook
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  2. Timothy P. Newsome
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Corresponding author

Correspondence to Timothy P. Newsome .

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Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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Marzook, N.B., Newsome, T.P. (2019). Construction and Isolation of Recombinant Vaccinia Virus Expressing Fluorescent Proteins. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

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