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Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis

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Vaccinia Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

This chapter describes the simple, rapid, and inexpensive preparation of template DNA from poxvirus-infected cells, plaques, or crude virus stocks for PCR amplification. This technique is reliable and robust and only requires centrifugation, detergent, and protease treatment. The resulting DNA template preparation is suitable for PCR amplification for screening viruses, cloning, transfection, and DNA sequencing.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA

    Rachel L. Roper

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  1. Rachel L. Roper
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Correspondence to Rachel L. Roper .

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Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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Roper, R.L. (2019). Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

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