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Ribosome Profiling of Vaccinia Virus-Infected Cells

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Vaccinia Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

Ribosome profiling is a method that determines genome-wide mRNA translation through measuring ribosome-protected mRNA fragments by deep sequencing. This method can be used to quantify gene expression at the translational level and precisely pinpoint ribosome loading onto mRNA with codon-level resolution. Genome-wide regulation of mRNA translation can also be determined if RNA-Sequencing (RNA-Seq) is carried out in parallel. Here, we describe a protocol for simultaneously performing ribosome profiling and RNA-Seq in cells infected with vaccinia virus.

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Acknowledgments

This work is supported, in part, by NIH grants to ZY, including a project of P20GM113117. This work is also supported, in part, by National Science Foundation of China grant NSFC81571988 to WQ.

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Authors and Affiliations

  1. Division of Biology, Kansas State University, Manhattan, KS, USA

    Yongquan Lin & Zhilong Yang

  2. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China

    Yongquan Lin & Wentao Qiao

Authors
  1. Yongquan Lin
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  2. Wentao Qiao
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  3. Zhilong Yang
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Corresponding author

Correspondence to Zhilong Yang .

Editor information

Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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Lin, Y., Qiao, W., Yang, Z. (2019). Ribosome Profiling of Vaccinia Virus-Infected Cells. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

  • eBook Packages: Springer Protocols

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