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Measurement of Specific IgG4 Anti-mouse Urine Antibodies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2020))

Abstract

IgG4 and its role in immune tolerance has been investigated widely. Symptom reduction and improved clinical outcomes in immunotherapy trials are associated with significant increases in allergen-specific IgG4 antibodies. Natural immune tolerance observed in beekeepers and cat owners has also been associated with elevated levels of bee venom and cat allergen-specific IgG4, respectively. Functionally, allergen-specific IgG4 has been shown to reduce the binding of IgE-allergen complexes to B cells, a key step in the initiation of the type 1 hypersensitivity allergic response. In laboratory animal allergy, IgG4 has been described as a “protective/blocking” antibody. However, a consensus on the exact relationship between exposure, IgG4, and tolerance has yet to be reached. In this chapter, we review the factors that require consideration when developing an ELISA for the quantification of allergen-specific IgG4.

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Author information

Authors and Affiliations

  1. Department of Population Health and Occupational Disease, NHLI @ Imperial College, London, UK

    Jennifer Canizales

Authors
  1. Jennifer Canizales
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Correspondence to Jennifer Canizales .

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Editors and Affiliations

  1. Core Facilities, St. George’s University of London, London, UK

    Penny Lympany

  2. Department of Occupational and Environmental Medicine, Imperial College London, London, UK

    Meinir G. Jones

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Canizales, J. (2019). Measurement of Specific IgG4 Anti-mouse Urine Antibodies. In: Lympany, P., Jones, M. (eds) Allergy. Methods in Molecular Biology, vol 2020. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9591-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9591-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9589-9

  • Online ISBN: 978-1-4939-9591-2

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