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In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

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Book cover Comparative Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2006))

Abstract

Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.

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Acknowledgments

C.H. and C.R. are grateful for the hard work and dedication of the surgeons, veterinarians, and animal care staff in the Division of Comparative Medicine, Behavioral Services Unit, and Surgical Services Unit at the Oregon National Primate Research Center (ONPRC) without which none of the animal work described here would have been possible. They would also like to thank the Assisted Reproductive Technology Core and the Endocrine Services Laboratory at the ONPRC for developing and optimizing the protocols presented in this chapter. Support at the ONPRC is provided by funding from the NIH grant P51 OD011092.

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Authors and Affiliations

  1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR, USA

    Cathy Ramsey & Carol Hanna

Authors
  1. Cathy Ramsey
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  2. Carol Hanna
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Corresponding author

Correspondence to Carol Hanna .

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Editors and Affiliations

  1. Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, NE, USA

    Jason R. Herrick

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Ramsey, C., Hanna, C. (2019). In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9566-0_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9565-3

  • Online ISBN: 978-1-4939-9566-0

  • eBook Packages: Springer Protocols

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