Abstract
The cynomolgus macaque (Macaca fascicularis) has contributed significantly as an animal model in a variety of biological systems including reproductive biology. The first reported delivery of a cynomolgus infant born through IVF was in 1984, and since then the use of assisted reproductive technologies (ARTs) in the cynomolgus macaque for modeling primate early embryonic development has evolved to include studies on gene editing in primate embryos and embryonic stem cell chimerism. The significance of the cynomolgus as a model of primate reproduction not only lies in the similarity of their reproductive physiology to the human but also in their availability, moderate size, and year-round utility with no seasonal breeding constraints. While many of the ARTs developed and refined in the rhesus also apply to the cynomolgus there are a number of variations that have proven effective in this species. The following chapter provides an overview of the ART methodologies that support the efficient production of M. fascicularis embryos, their cryopreservation and their transfer to recipient females for the establishment of pregnancy.
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Curnow E, Ryan J, Saunders D, Hayes E (2010) In vitro developmental potential of macaque oocytes, derived from unstimulated ovaries, following maturation in the presence of glutathione ethyl ester. Hum Reprod 25:2465–2474
Curnow EC, Ryan JP, Saunders DM, Hayes ES (2011) Primate model of metaphase I oocyte in vitro maturation and the effects of a novel glutathione donor on maturation, fertilization, and blastocyst development. Fertil Steril 95:1235–1240
Hayes E, Curnow E, Trounson A, Danielson L, Unemori E (2004) Implantation and pregnancy following in vitro fertilization and the effect of recombinant human relaxin administration in Macaca fascicularis. Biol Reprod 71:1591–1597
Curnow E, Kuleshova L, Shaw J, Hayes E (2002) Comparison of slow-and rapid-cooling protocols for early-cleavage-stage Macaca fascicularis embryos. Am J Primatol 58:169–174
Balmaceda JP, Heitman TO, Garcia MR, Pauerstein CJ, Pool TB (1986) Embryo cryopreservation in cynomolgus monkeys. Fertil Steril 45:403–406
Dukelow WR, Brüggemann S (1979) Characteristics of the menstrual cycle in nonhuman primates. II. Ovulation and optimal mating times in macaques. J Med Primatol 8(2):79–87
Acknowledgments
E.C.C. and E.S.H. would like to thank the veterinary and animal care staff at the Washington National Primate Research Center, particularly Cliff Astley for surgery support and Clayton Ferrier for assistance in working with the macaques for cycle detection, gamete collection, and embryo transfer. The authors thank the WaNPRC’s Tissue Distribution Program for their assistance with the collection of reproductive tissues. Studies at the WaNPRC were supported by NIH grant P51 OD010425.
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Curnow, E., Hayes, E. (2019). In Vitro Culture of Embryos from the Cynomolgus Macaque (Macaca fascicularis). In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_22
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DOI: https://doi.org/10.1007/978-1-4939-9566-0_22
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