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The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer

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Comparative Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2006))

Abstract

Advances in embryo technologies in the domestic dog have made significant strides in the past decade. This progress has been spurred by interests in taking advantage of the dog as a biomedical research model for human and companion animal medicine, developing assisted reproductive technologies to manage genetic diversity in endangered canids maintained ex situ, and improving breeding in rare or working breeds of dogs. Here, we focus on recent advancements and techniques for collection of in vivo-matured oocytes, in vitro fertilization (IVF), in vitro culture of early (≤8-cell) and advanced stage (≥16-cell) embryos, and embryo transfer.

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Authors and Affiliations

  1. Smithsonian Conservation Biology Institute, Front Royal, VA, USA

    J. B. Nagashima & N. Songsasen

  2. Baker Institute for Animal Health, College of Veterinary Medicine, Ithaca, NY, USA

    A. J. Travis

  3. Atkinson Center for a Sustainable Future, Cornell University, Ithaca, NY, USA

    A. J. Travis

Authors
  1. J. B. Nagashima
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  2. A. J. Travis
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  3. N. Songsasen
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Corresponding author

Correspondence to J. B. Nagashima .

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Editors and Affiliations

  1. Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, NE, USA

    Jason R. Herrick

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Nagashima, J.B., Travis, A.J., Songsasen, N. (2019). The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9566-0_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9565-3

  • Online ISBN: 978-1-4939-9566-0

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