Phloem pp 421-431 | Cite as

Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System

  • Chao Xia
  • Yi Zheng
  • Jing Huang
  • Zhangjun Fei
  • Cankui ZhangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)


Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a prerequisite for elucidating their physiological functions. Nicotiana benthamiana can be used as a scion to graft on a tomato (Solanum lycopersicum) rootstock. Thereby, shoot-to-root mobile N. benthamiana mRNAs transported via the phloem can be identified from the root of the tomato rootstock. Due to the close relationship and similar genome sequences of the two species, stringent informatics procedures should be applied to avoid false identification. This heterograft system can be used to study physiological processes associated with mRNAs that are mobile under either normal or adverse growth condition.

Key words

Phloem transport Heterograft Mobile mRNA Systemic signaling RNA-Seq Long-distance transport 



This work was supported by funds from Purdue University as part of AgSEED Crossroads funding to support Indiana’s Agriculture and Rural Development (to C. Z.), National Key Research and Development Project of China (2016YFD0101206), and the US National Science Foundation (IOS-1339287 and IOS-1539831).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Chao Xia
    • 1
    • 2
  • Yi Zheng
    • 3
  • Jing Huang
    • 2
  • Zhangjun Fei
    • 3
    • 4
  • Cankui Zhang
    • 5
    Email author
  1. 1.Maize Research Institute, Sichuan Agricultural UniversityChengduChina
  2. 2.Department of Agronomy and Purdue Center for Plant BiologyPurdue UniversityWest LafayetteUSA
  3. 3.Boyce Thompson Institute, Cornell UniversityIthacaUSA
  4. 4.U.S. Department of Agriculture-Agricultural Research Service Robert W. Holley Center for Agriculture and HealthIthacaUSA
  5. 5.Department of Agronomy and Purdue Center for Plant BiologyPurdue UniversityWest LafayetteUSA

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