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In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate

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Phloem

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2014))

Abstract

Opportunities and challenges in the use of radiotracers to measure phloem transport are discussed, with an emphasis on noninvasive techniques to trace photoassimilate, carbon’s short-lived isotope 11C, and an eye to pitfalls and traps to avoid. We discuss in turn the rationale for using tracers, the limitations and complications with using short-lived radiotracers like 11C, the physics of decay and detection that need to be known when data are interpreted, and methods of analysis.

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Author information

Authors and Affiliations

  1. New Zealand Institute for Plant and Food Research, Motueka Research Centre, Motueka, New Zealand

    Michael R. Thorpe

  2. New Zealand Institute for Plant and Food Research, Motueka Research Centre, Motueka, New Zealand

    Peter E. H. Minchin

Authors
  1. Michael R. Thorpe
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  2. Peter E. H. Minchin
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Correspondence to Michael R. Thorpe .

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Editors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, China

    Johannes Liesche

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Thorpe, M.R., Minchin, P.E.H. (2019). In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate. In: Liesche, J. (eds) Phloem. Methods in Molecular Biology, vol 2014. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9562-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9562-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9561-5

  • Online ISBN: 978-1-4939-9562-2

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