Phloem pp 177-194 | Cite as

In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate

  • Michael R. ThorpeEmail author
  • Peter E. H. Minchin
Part of the Methods in Molecular Biology book series (MIMB, volume 2014)


Opportunities and challenges in the use of radiotracers to measure phloem transport are discussed, with an emphasis on noninvasive techniques to trace photoassimilate, carbon’s short-lived isotope 11C, and an eye to pitfalls and traps to avoid. We discuss in turn the rationale for using tracers, the limitations and complications with using short-lived radiotracers like 11C, the physics of decay and detection that need to be known when data are interpreted, and methods of analysis.

Key words

111418Radiotracers Carbon-11 Carbon-14 Radiocarbon Carbon allocation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.New Zealand Institute for Plant and Food ResearchMotueka Research CentreMotuekaNew Zealand
  2. 2.New Zealand Institute for Plant and Food ResearchMotueka Research CentreMotuekaNew Zealand

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