Abstract
Proteomics is an excellent technique for detecting proteins involved in plant responses infected with the Citrus tristeza virus. Here we describe the process to extract proteins to obtain two-dimensional gels with a large number of spots, well distributed, and with good quality. The extraction process that associates successive washes with ultrasonication steps proved to be efficient for the detection of proteins. With this technique it was possible to obtain clear gels which were detected in about 600 spots distributed throughout the gel without the presence of drag.
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Acknowledgments
This work was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq), process N° 304536/2015-6.
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Dória, M.S., Pirovani, C.P. (2019). Proteomic Response of Host Plants to Citrus tristeza virus. In: Catara, A., Bar-Joseph, M., Licciardello, G. (eds) Citrus Tristeza Virus. Methods in Molecular Biology, vol 2015. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9558-5_15
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DOI: https://doi.org/10.1007/978-1-4939-9558-5_15
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