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Chromatin Immunoprecipitation Techniques in Neuropsychiatric Research

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Book cover Psychiatric Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2011))

Abstract

Neuropsychiatric disorders are highly prevalent (e.g., affecting children 2–8 years old at a rate of 14%). Many of these disorders are highly heritable such as major depressive disorder and schizophrenia. Despite this, genome-wide association has failed to identify gene(s) significantly associated with diagnostic status suggesting a strong role for environmental factors and the epigenome. From a molecular standpoint, the study of DNA-protein interactions yields fruitful information regarding the regulation of cellular processes above the level of the nucleotide sequence. Understanding chromatin dynamics may continue to explain individual variation to environmental perturbation and subsequent behavioral response. Chromatin immunoprecipitation (ChIP) techniques have allowed for probing of epigenetic effectors at specific regions of the genome. The following article reviews the current techniques and considerations when incorporation ChIP into neuropsychiatric models.

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Acknowledgments

The authors would like to thank both Brian B Griffiths and Amanda MK Madden for technical assistance as well as the University of Massachusetts Boston for startup funding (RGH).

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Authors and Affiliations

  1. Department of Psychology, University of Massachusetts, Boston, Boston, MA, USA

    Andrew A. Bartlett & Richard G. Hunter

  2. Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY, USA

    Richard G. Hunter

Authors
  1. Andrew A. Bartlett
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  2. Richard G. Hunter
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Corresponding author

Correspondence to Richard G. Hunter .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Firas H. Kobeissy

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Bartlett, A.A., Hunter, R.G. (2019). Chromatin Immunoprecipitation Techniques in Neuropsychiatric Research. In: Kobeissy, F. (eds) Psychiatric Disorders. Methods in Molecular Biology, vol 2011. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9554-7_36

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  • DOI: https://doi.org/10.1007/978-1-4939-9554-7_36

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9553-0

  • Online ISBN: 978-1-4939-9554-7

  • eBook Packages: Springer Protocols

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