Abstract
The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.
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Acknowledgments
This paper is published with the permission of the director of KEMRI. F.H.A.O. is supported by an MRC/DFID African Research Leader Award jointly funded by the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement (MR/L00450X/1); an EDCTP Senior Fellowship (TMA 2015 SF—1001); and a Sofja Kovalevskaja Award from the Alexander von Humboldt Foundation (3·2—1184811—KEN—SKP). L.M.M. is supported by the DELTAS Africa Initiative [DEL-15-003]. The DELTAS Africa Initiative is an independent funding scheme of the African Academy of Sciences (AAS)’s Alliance for Accelerating Excellence in Science in Africa (AESA) and supported by the New Partnership for Africa’s Development Planning and Coordinating Agency (NEPAD Agency) with funding from the Wellcome Trust [107769/Z/10/Z] and the UK government. The views expressed in this publication are those of the author(s) and not necessarily those of AAS, NEPAD Agency, Wellcome Trust, or the UK government. RKK and JT are supported by a Wellcome Trust Strategic Award (107499/Z/15/Z). GK was jointly supported by the MRC/DFID African Research Leader Award (MR/L00450X/1) and the DELTAS Africa Initiative [DEL-15-003].
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Murungi, L.M., Kimathi, R.K., Tuju, J., Kamuyu, G., Osier, F.H.A. (2019). Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol. In: Ariey, F., Gay, F., Ménard, R. (eds) Malaria Control and Elimination. Methods in Molecular Biology, vol 2013. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9550-9_6
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DOI: https://doi.org/10.1007/978-1-4939-9550-9_6
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