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Mouse Models of Yersiniosis

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Pathogenic Yersinia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2010))

Abstract

Yersiniosis is common foodborne gastrointestinal disease caused by the enteric pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis. The mouse model of oral infection serves as a useful tool to study enteropathogenic Yersinia infection in mammals. The following protocol describes two distinct oral infection methods: the commonly used oral gavage method in which the bacterial inoculum is instilled directly into the mouse stomach using a feeding needle, and an alternative method in which mice are fed bread soaked with Yersinia culture.

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Acknowledgments

We thank Susannah McKay and Daniel Portnoy for technical advice in establishing the bread feeding model in Yersinia. D.H.-R. is supported by a Ford Foundation fellowship. The mouse infection data shown are taken from the work supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Numbers R21AI099747 and R01AI119082 (to V.A.).

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Authors and Affiliations

  1. Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA, USA

    Diana Hooker-Romero, Yahan Wei & Victoria Auerbuch

  2. Department of Molecular, Cell and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA, USA

    Leah Schwiesow

Authors
  1. Diana Hooker-Romero
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  2. Leah Schwiesow
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  3. Yahan Wei
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  4. Victoria Auerbuch
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Corresponding author

Correspondence to Victoria Auerbuch .

Editor information

Editors and Affiliations

  1. Paul G Allen School for Global Animal Health, Washington State University, Pullman, WA, USA

    Viveka Vadyvaloo

  2. Department of Microbiolgy and Immunology and Center for Predictive Medicine in Biodefense and Emerging Infectious Diseases, University of Louisville School of Medicine, Louisville, KY, USA

    Matthew B. Lawrenz

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Hooker-Romero, D., Schwiesow, L., Wei, Y., Auerbuch, V. (2019). Mouse Models of Yersiniosis. In: Vadyvaloo, V., Lawrenz, M. (eds) Pathogenic Yersinia. Methods in Molecular Biology, vol 2010. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9541-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9541-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9540-0

  • Online ISBN: 978-1-4939-9541-7

  • eBook Packages: Springer Protocols

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