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Establishing Cell Culture-Based Experimental Setups for Proximity Labeling Using Ascorbate Peroxidase (APEX)

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Proximity Labeling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2008))

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Abstract

Proximity labeling by ascorbate peroxidase (APEX) requires appropriate experimental setups that generate sufficient signal over background as a prerequisite for downstream analyses by mass spectrometry. Cell culture-based systems are easily accessible, yet, for proximity labeling of small structures must be carefully optimized in order to give satisfying results. How to establish and characterize APEX cell lines will be the topic of this chapter.

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Acknowledgment

I thank Bianca Schrul and members of the Mick lab for critically reading the manuscript.

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Authors and Affiliations

  1. Center for Human and Molecular Biology (ZHMB), Medical Biochemistry and Molecular Biology, Saarland University School of Medicine, 66421, Homburg, Germany

    David U. Mick

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  1. David U. Mick
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Correspondence to David U. Mick .

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Editors and Affiliations

  1. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany

    Murat Sunbul  & Andres Jäschke  & 

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Mick, D.U. (2019). Establishing Cell Culture-Based Experimental Setups for Proximity Labeling Using Ascorbate Peroxidase (APEX). In: Sunbul, M., Jäschke, A. (eds) Proximity Labeling. Methods in Molecular Biology, vol 2008. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9537-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9537-0_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9536-3

  • Online ISBN: 978-1-4939-9537-0

  • eBook Packages: Springer Protocols

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