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In Vitro Measurement of H2S-Mediated Vasoactive Responses

  • Sona CacanyiovaEmail author
  • Andrea Berenyiova
Protocol
  • 382 Downloads
Part of the Methods in Molecular Biology book series (MIMB, volume 2007)

Abstract

Isolated tissue chamber bath system and wire myograph were developed for “in vitro” investigation of vasoactive responses on isolated arteries from a variety of animal species and vascular beds. The chapter characterizes the main principles of mechanical measurement of the changes in isometric tension of vascular smooth muscles in isolated rat thoracic aorta and superior mesenteric artery and describes several protocols on how to investigate vasoactive properties of hydrogen sulfide (H2S) from the point of view of its mutual interaction with NO. Several methodological advances, results, and their interpretations in the context of the general knowledge are described. In the protocols the approach on how to study the vasoactive modulatory as well as direct action of H2S and mutual interaction of H2S with nitroso compounds, lipids, and endogenously produced NO is described.

Key words

Isolated artery Vasoactivity NaHS Na2Nitrosothiol NO synthase Wistar rat SHR 

Notes

Acknowledgments

Financial support by Slovak grants VEGA 2/0103/18, APVV-15-0565, and APVV-15-0371 is gratefully acknowledged.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Normal and Pathological Physiology, Center of Experimental MedicineSlovak Academy of SciencesBratislavaSlovakia

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