Abstract
Vascular myography is an in vitro technique used to examine functional responses of isolated blood vessels. This classical pharmacological technique has been in use for over a century. The assay technique studies changes in isometric tone of large and small vessels, arteries and veins, and tissues from genetic or disease models. This chapter describes the apparatus required, tissue collection methods, and the mounting of the tissues in the chambers of both large organ baths and the small vessel myograph. Considerations of the experimental conditions and design are discussed as well as the analysis of the collected data.
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Acknowledgments
Reproduced traces shown are adapted from original LabChart recordings generated by Ms. Suzan Yildiz and Mr. Jafer Al Qaeisoom.
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Hart, J. (2019). Vascular Myography to Examine Functional Responses of Isolated Blood Vessels. In: Bełtowski, J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9528-8_15
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DOI: https://doi.org/10.1007/978-1-4939-9528-8_15
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