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Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

  • Joanne HartEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2007)

Abstract

Vascular myography is an in vitro technique used to examine functional responses of isolated blood vessels. This classical pharmacological technique has been in use for over a century. The assay technique studies changes in isometric tone of large and small vessels, arteries and veins, and tissues from genetic or disease models. This chapter describes the apparatus required, tissue collection methods, and the mounting of the tissues in the chambers of both large organ baths and the small vessel myograph. Considerations of the experimental conditions and design are discussed as well as the analysis of the collected data.

Key words

Myography Blood vessels Endothelial function Nitric oxide bioavailability 

Notes

Acknowledgments

Reproduced traces shown are adapted from original LabChart recordings generated by Ms. Suzan Yildiz and Mr. Jafer Al Qaeisoom.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Medicine and Health, School of MedicineUniversity of SydneyCamperdownAustralia

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