Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

  • Joanne HartEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2007)


Vascular myography is an in vitro technique used to examine functional responses of isolated blood vessels. This classical pharmacological technique has been in use for over a century. The assay technique studies changes in isometric tone of large and small vessels, arteries and veins, and tissues from genetic or disease models. This chapter describes the apparatus required, tissue collection methods, and the mounting of the tissues in the chambers of both large organ baths and the small vessel myograph. Considerations of the experimental conditions and design are discussed as well as the analysis of the collected data.

Key words

Myography Blood vessels Endothelial function Nitric oxide bioavailability 



Reproduced traces shown are adapted from original LabChart recordings generated by Ms. Suzan Yildiz and Mr. Jafer Al Qaeisoom.


  1. 1.
    Mulvany MJ, Halpern W (1977) Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res 41(1):19–26CrossRefGoogle Scholar
  2. 2.
    Leo CH et al (2012) Endothelium-dependent nitroxyl-mediated relaxation is resistant to superoxide anion scavenging and preserved in diabetic rat aorta. Pharmacol Res 66(5):383–391CrossRefGoogle Scholar
  3. 3.
    Jones RL, Woodward DF (2011) Interaction of prostanoid EP(3) and TP receptors in guinea-pig isolated aorta: contractile self-synergism of 11-deoxy-16,16-dimethyl PGE(2). Br J Pharmacol 162(2):521–531CrossRefGoogle Scholar
  4. 4.
    Hart JL, Sobey CG, Woodman OL (1995) Cholesterol feeding enhances vasoconstrictor effects of products from rabbit polymorphonuclear leukocytes. Am J Phys 269(1 Pt 2):H1–H6Google Scholar
  5. 5.
    McPherson GA et al (1999) Functional and electrophysiological effects of a novel imidazoline-based K(ATP) channel blocker, IMID-4F. Br J Pharmacol 128(8):1636–1642CrossRefGoogle Scholar
  6. 6.
    Berkenboom G et al (1989) Comparison of responses to acetylcholine and serotonin on isolated canine and human coronary arteries. Cardiovasc Res 23(9):780–787CrossRefGoogle Scholar
  7. 7.
    Leo CH, Hart JL, Woodman OL (2011) Impairment of both nitric oxide-mediated and EDHF-type relaxation in small mesenteric arteries from rats with streptozotocin-induced diabetes. Br J Pharmacol 162(2):365–377CrossRefGoogle Scholar
  8. 8.
    Leo CH, Hart JL, Woodman OL (2011) 3′,4′-Dihydroxyflavonol reduces superoxide and improves nitric oxide function in diabetic rat mesenteric arteries. PLoS One 6(6):e20813CrossRefGoogle Scholar
  9. 9.
    Al-Magableh MR et al (2014) Hydrogen sulfide protects endothelial nitric oxide function under conditions of acute oxidative stress in vitro. Naunyn Schmiedeberg’s Arch Pharmacol 387(1):67–74CrossRefGoogle Scholar
  10. 10.
    Papapetropoulos A, Whiteman M, Cirino G (2015) Pharmacological tools for hydrogen sulphide research: a brief, introductory guide for beginners. Br J Pharmacol 172(6):1633–1637CrossRefGoogle Scholar
  11. 11.
    Stork AP, Cocks TM (1994) Pharmacological reactivity of human epicardial coronary arteries: characterization of relaxation responses to endothelium-derived relaxing factor. Br J Pharmacol 113(4):1099–1104CrossRefGoogle Scholar
  12. 12.
    Al-Magableh MR, Hart JL (2011) Mechanism of vasorelaxation and role of endogenous hydrogen sulfide production in mouse aorta. Naunyn Schmiedeberg’s Arch Pharmacol 383(4):403–413CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Medicine and Health, School of MedicineUniversity of SydneyCamperdownAustralia

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