Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

  • Marcin UfnalEmail author
  • Artur Nowinski
Part of the Methods in Molecular Biology book series (MIMB, volume 2007)


Increasing evidence suggests that hydrogen sulfide (H2S) is involved in brain mechanisms regulating the functions of the circulatory system. This appears to be mediated by cardiovascular centers located in the central nervous system. This chapter describes techniques of acute and chronic infusions into the brain cardiovascular centers in rats. Rats may be implanted either acutely or chronically with a cannula inserted into a selected cardiovascular center according to the stereotaxic coordinates. The cannula allows for the administration of the investigated compounds into a selected cardiovascular center.

Key words

Hydrogen sulfide Central infusions Intracerebroventricular Circumventricular organs Paraventricular nucleus Rostral ventrolateral medulla Caudal ventrolateral medulla 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Centre for Preclinical Research, Department of Experimental Physiology and PathophysiologyMedical University of WarsawWarsawPoland

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