Pharmacological Tools for the Study of H2S Contribution to Angiogenesis
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Recently, hydrogen sulfide (H2S) has been characterized as an endogenous mediator able to control a series of cellular and tissue functions relevant for tissue homeostasis and repair such as angiogenesis. This chapter describes the tools and their use in a set of angiogenesis assays performed by using cultured endothelial cells in order to study the relevance of exogenous or endogenous H2S production and release during the occurrence of angiogenesis.
Key wordsAngiogenesis Endothelial cells Hydrogen sulfide Cystathionine γ-lyase Proliferation Migration Chemoinvasion Permeability Gelatinase
Part of this work was funded by Italian Space Agency (project “Tissue Repair in Microgravity” ASI N. 2013-090-R.O).
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