Abstract
Recently, hydrogen sulfide (H2S) has been characterized as an endogenous mediator able to control a series of cellular and tissue functions relevant for tissue homeostasis and repair such as angiogenesis. This chapter describes the tools and their use in a set of angiogenesis assays performed by using cultured endothelial cells in order to study the relevance of exogenous or endogenous H2S production and release during the occurrence of angiogenesis.
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References
Chung AS, Ferrara N (2011) Developmental and pathological angiogenesis. Ann Rev Cell Dev Biol 27:563–584
Welti J, Loges S, Dimmeler S, Carmeliet P (2013) Recent molecular discoveries in angiogenesis and antiangiogenic therapies in cancer. J Clin Invest 123(8):3190–3200
Ferrara N, Adamis AP (2016) Ten years of anti-vascular endothelial growth factor therapy. Nat Rev Drug Discov 15(6):385–403. https://doi.org/10.1038/nrd.2015.17
Bachetti T, Morbidelli L (2000) Endothelial cells in culture: a model for studying vascular functions. Pharmacol Res 42:9–19
Ziche M, Morbidelli L, Masini E et al (1994) Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P. J Clin Invest 94:2036–2044
Morbidelli L, Chang C-H, Douglas JG et al (1996) Nitric oxide mediates mitogenic effect of VEGF on coronary venular endothelium. Am J Phys 39(1):H411–H4156
Morbidelli L, Pyriochou A, Filippi S et al (2010) The soluble guanylyl cyclase inhibitor NS-2028 reduces vascular endothelial growth factor-induced angiogenesis and permeability. Am J Physiol Regul Integr Comp Physiol 298(3):R824–R832
Terzuoli E, Monti M, Vellecco V et al (2015) Characterization of zofenoprilat as an inducer of functional angiogenesis through increased H2S availability. Br J Pharmacol 172(12):2961–2973. https://doi.org/10.1111/bph.13101
Monti M, Terzuoli E, Ziche M, Morbidelli L (2016) H2S dependent and independent anti-inflammatory activity of zofenoprilat in cells of the vascular wall. Pharmacol Res 113(Pt A):426–437. https://doi.org/10.1016/j.phrs.2016.09.017
Kimura H (2011) Hydrogen sulfide: its production, release and functions. Amino Acids 41(1):113–121. https://doi.org/10.1007/s00726-010-0510-x
Wallace JL, Wang R (2015) Hydrogen sulphide-based therapeutics: exploiting an unique but ubiquitous gasotransmitter. Nat Rev 14:329–345
Yang G, Wu L, Jiang B et al (2008) H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase. Science 322(5901):587–590. https://doi.org/10.1126/science.1162667
Yang G, Wang R (2015) H2S and blood vessels: an overview. Handb Exp Pharmacol 230:85–110
Katsouda A, Bibli SI, Pyriochou A et al (2016) Regulation and role of endogenously produced hydrogen sulfide in angiogenesis. Pharmacol Res 113(Pt A):175–185. https://doi.org/10.1016/j.phrs.2016.08.026
Li L, Whiteman M, Guan YY, Neo KL et al (2008) Characterization of a novel, water-soluble hydrogen sulfide-releasing molecule (GYY4137): new insights into the biology of hydrogen sulfide. Circulation 117(18):2351–2360. https://doi.org/10.1161/CIRCULATIONAHA.107.753467
Szczesny B, Módis K, Yanagi K et al (2014) AP39, a novel mitochondria-targeted hydrogen sulfide donor, stimulates cellular bioenergetics, exerts cytoprotective effects and protects against the loss of mitochondrial DNA integrity in oxidatively stressed endothelial cells in vitro. Nitric Oxide 41:120–130. https://doi.org/10.1016/j.niox.2014.04.008
Monti M, Solito R, Puccetti L et al (2014) Protective effects of novel metal-nonoates on the cellular components of the vascular system. J Pharmacol Exp Ther 351(3):500–509. https://doi.org/10.1124/jpet.114.218404
Liu C, Pan J, Li S et al (2011) Capture and visualization of hydrogen sulfide via a fluorescent probe. Angew Chem Int Ed Engl 50(44):10327–10329
Acknowledgments
Part of this work was funded by Italian Space Agency (project “Tissue Repair in Microgravity” ASI N. 2013-090-R.O).
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Morbidelli, L., Monti, M., Terzuoli, E. (2019). Pharmacological Tools for the Study of H2S Contribution to Angiogenesis. In: Bełtowski, J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9528-8_11
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DOI: https://doi.org/10.1007/978-1-4939-9528-8_11
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