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SMC Complexes pp 169-180 | Cite as

Dissecting DNA Compaction by the Bacterial Condensin MukB

  • Rupesh Kumar
  • Soon Bahng
  • Kenneth J. MariansEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2004)

Abstract

Condensins in bacteria are one of the most important factors involved in the organization of long threads of DNA into compact chromosomes. The organization of DNA by condensins is vital to many DNA transactions including DNA repair and chromosome segregation. Although some of the activities of condensins are well studied, the mechanism of the overall process executed by condensins, DNA compaction, remains unclear. Here, we describe some of the methods used routinely in our laboratory to understand the mechanism of DNA compaction by Escherichia coli condensin MukB.

Key words

MukB Condensins SMC Chromatin DNA condensation DNA looping DNA bridging DNA supercoiling 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Molecular Biology ProgramMemorial Sloan Kettering Cancer CenterNew YorkUSA

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