Abstract
Silver and gold nanoparticles (NPs) attract great attention nowadays because of their unique characteristics that entitle them for various biomedical applications. However, there is still a need for successful green synthesis methods for these two metal NPs, especially in a hybrid form, as well-established protocols. On the other hand, chitosan (Cs) is a polysaccharide of great promise for green synthesis of metal NPs, especially in the presence of some plant/fruit extracts. Together, Cs and the appropriate natural products in the extracts play the roles of both capping and reducing agents toward the green synthesis and stabilization of the silver/gold hybrid NPs. In this chapter, we introduce a well-established protocol for the green synthesis of Cs-Ag/Au hybrid NPs which could incorporate a therapeutic agent. In this approach, Cs plays the role of a carrier for the therapeutic agent, in addition to its capping/reducing contributions.
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References
Guzmán MG, Dille J, Godet S (2009) Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biomol Eng 2:3
Baker C et al (2005) Synthesis and antibacterial properties of silver nanoparticles. J Nanosci Nanotechnol 5(2):244–249
Cao YW, Rongchao J, Chad AM (2001) DNA-modified core−shell Ag/Au nanoparticles. J Am Chem Soc 123(32):7961–7962
Matejka P et al (1992) The role of triton X-100 as an adsorbate and a molecular spacer on the surface of silver colloid: a surface-enhanced Raman scattering study. J Phys Chem 96(3):1361–1366
Pileni MP (2000) Fabrication and physical properties of self-organized silver nanocrystals. Pure Appl Chem 72(1–2):53–65
Henglein A (2001) Reduction of Ag (CN) 2-on silver and platinum colloidal nanoparticles. Langmuir 17(8):2329–2333
Esumi K, Keiichi M, Kanjiro T (1995) Preparation of rodlike gold particles by UV irradiation using cationic micelles as a template. Langmuir 11(9):3285–3287
Klaus T et al (1999) Silver-based crystalline nanoparticles, microbially fabricated. Proc Natl Acad Sci 96(24):13611–13614
Ahmad A et al (2003) Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, rhodococcus species. Nanotechnology 14(7):824
Beattie IR, Richard GH (2011) Silver and gold nanoparticles in plants: sites for the reduction to metal. Metallomics 3(6):628–632
Song JY, Beom SK (2008) Biological synthesis of bimetallic Au/Ag nanoparticles using persimmon (Diospyros kaki) leaf extract. Korean J Chem Eng 25(4):808–811
Kumar V, Subhash CY, Sudesh KY (2010) Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol Biotechnol 85(10):1301–1309
Dubey SP et al (2010) Bioprospective of Sorbus aucuparia leaf extract in development of silver and gold nanocolloids. Colloids Surf B: Biointerfaces 80(1):26–33
Das RK et al (2011) Synthesis of gold nanoparticles using aqueous extract of Calotropis procera latex. Mater Lett 65(4):610–613
Ahmad N et al (2010) Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B: Biointerfaces 81(1):81–86
Sedki M et al (2015) Phytosynthesis of silver–reduced graphene oxide (Ag–RGO) nanocomposite with an enhanced antibacterial effect using Potamogeton pectinatus extract. RSC Adv 5(22):17358–17365
AbdelHamid AA et al (2013) Phytosynthesis of Au, Ag, and Au–Ag bimetallic nanoparticles using aqueous extract of sago pondweed (Potamogeton pectinatus L.). ACS Sustain Chem Eng 1(12):1520–1529
Muzzarelli RAA (2009) Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydr Polym 76(2):167–182
Thakur VK, Manju KT (2014) Recent advances in graft copolymerization and applications of chitosan: a review. ACS Sustain Chem Eng 2(12):2637–2652
An J et al (2013) Electrochemical study and application on rutin at chitosan/graphene films modified glassy carbon electrode. J Pharmaceut Anal 3(2):102–108
Bhumkar DR et al (2007) Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of insulin. Pharm Res 24(8):1415–1426
Huang H, Xiurong Y (2004) Synthesis of chitosan-stabilized gold nanoparticles in the absence/presence of tripolyphosphate. Biomacromolecules 5(6):2340–2346
El-Sherbiny IM et al (2016) Newly developed chitosan-silver hybrid nanoparticles: biosafety and apoptosis induction in HepG2 cells. J Nanopart Res 18(7):1–13
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El-Sherbiny, I.M., Sedki, M. (2019). Green Synthesis of Chitosan-Silver/Gold Hybrid Nanoparticles for Biomedical Applications. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_7
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DOI: https://doi.org/10.1007/978-1-4939-9516-5_7
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