Abstract
Solid lipid nanoparticles (SLNs) have been extensively investigated for effective delivery of both hydrophilic and lipophilic drugs by topical route. There are several scalable techniques for the preparation of SLNs such as homogenization, microemulsion template, and solvent emulsification diffusion. This chapter describes step-wise methodology for the preparation and characterization of SLNs using solvent emulsification diffusion method. Tretinoin, a lipophilic entity, was chosen as a model drug. The critical aspects and the important interpretations with respect to the preparation and characterization of SLNs are reported in “Notes” section.
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References
Liu J, Hu W, Chen H et al (2007) Isotretinoin-loaded solid lipid nanoparticles with skin targeting for topical delivery. Int J Pharm 328:191–195
Kumar VV, Chandrasekar D, Ramakrishna S et al (2007) Development and evaluation of nitrendipine loaded solid lipid nanoparticles: influence of wax and glyceride lipids on plasma pharmacokinetics. Int J Pharm 335:167–175
You J, Wan F, Cui FD et al (2007) Preparation and characteristic of vinorelbine bitartrate-loaded solid lipid nanoparticles. Int J Pharm 343:270–276
Ma QH, Xia Q, Lu YY et al (2007) Preparation of tea polyphenols loaded solid lipid nanoparticles based on the phase behaviors of hot microemulsions. Solid State Phenom 121:705–708
Ugazio E, Cavalli R, Gasco MR (2002) Incorporation of cyclosporin a in solid lipid nanoparticles (SLN). Int J Pharm 241:341–344
Zhang D, Tan T, Gao L (2006) Preparation of oridonin-loaded solid lipid nanoparticles and studies of them in vitro and in vivo. Nanotechnology 17:5821–5828
Hou DZ, Xie CS, Huang KJ (2003) The production and characteristics of solid lipid nanoparticles (SLNs). Biomaterials 24:1781–1785
Luo YF, Chen DW, Ren LX et al (2006) Solid lipid nanoparticles for enhancing vinpocetine’s oral bioavailability. J Control Release 114:53–59
Utada AS, Lorenceau E, Link DR et al (2005) Microcapillary device monodisperse double emulsions generated from a microcapillary device. Science 308:537–541
Liu J, Gong T, Wang C et al (2007) Solid lipid nanoparticles loaded with insulin by sodium cholate-phosphatidylcholine- based mixed micelles: preparation and characterization. Int J Pharm 340:153–162
Zhang N, Ping Q, Huang G et al (2006) Lectin modified solid lipid nanoparticles as carriers for oral administration of insulin. Int J Pharm 327:153–159
Pandey R, Sharma S, Khuller GK (2005) Oral solid lipid nanoparticle based antitubercular chemotherapy. Tuberculosis 85:415–420
Hu FQ, Hong Y, Yuan H (2004) Preparation and characterization of solid lipid nanoparticles containing peptide. Int J Pharm 273:29–35
Shah KA, Date AA, Joshi MD, Patravale VB (2007) Solid lipid nanoparticles (SLN) of tretinoin: potential in topical delivery. Int J Pharm 345(1–2):163–171
Trotta M, Debernardi F, Caputo O (2003) Preparation of solid lipid nanoparticles by a solvent emulsification–diffusion technique. Int J Pharm 257:153–160
Joshi MD, Patravale VB (2006) Formulation and evaluation of nanostructured lipid carrier (NLC) based gel of valdecoxib. Drug Dev Ind Pharm 32:911–918
Pattani AS, Mandawgade SD, Patravale VB (2006) Development and comparative anti-microbial evaluation of lipid nanoparticles and nanoemulsion of Polymyxin B. J Nanosci Nanotechnol 6:1–5
Nanosep MF & Nanosep® Centrifugal devices (2017). https://shop.pall.com/INTERSHOP/web/WFS/PALL-PALLUS-Site/en_US/-/USD/ViewProduct-Start?SKU=gri78m16&CatalogID=Laboratory. Accessed 25 Feb 2017
Rohrs BR (2001) Dissolution method development for poorly soluble compounds. Dissolut Technol 8(3):1–5
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Patravale, V.B., Mirani, A.G. (2019). Preparation and Characterization of Solid Lipid Nanoparticles-Based Gel for Topical Delivery. In: Weissig, V., Elbayoumi, T. (eds) Pharmaceutical Nanotechnology. Methods in Molecular Biology, vol 2000. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9516-5_20
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DOI: https://doi.org/10.1007/978-1-4939-9516-5_20
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